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課程名稱 |
植物基因轉殖
PLANT GENE TRANSFER |
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開課學期 |
99-1 |
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授課對象 |
生物資源暨農學院 園藝暨景觀學系 |
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授課教師 |
黃鵬林 |
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課號 |
HORT5024 |
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課程識別碼 |
628 U1560 |
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班次 |
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學分 |
3 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期三2,3,4(9:10~12:10) |
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上課地點 |
園藝R106 |
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備註 |
第二群必選，三選一。
總人數上限：30人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/991plant_transfer |
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課程簡介影片 |
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核心能力關聯 |
本課程尚未建立核心能力關連 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
1. Introduction
2. Agrobacterium tumefaciens
3. Agrobacterium-mediated transformation
4. DNA-mediated gene transfer
5. Alternative DNA transfer methods
6. Virus vectors and their potential application in plant genetic engineering
7. Genetic manipulation of the chloroplast genome
8. Reporter genes
9. Antisense RNA technology and ribozyme
10. Manipulation of floral pigmentation genes
11. Herbicide resistance
12. Strategies for disease resistance in plants
13. Molecular strategy to modify genes involved in ethylene biosynthesis |
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課程目標 |
本課程以生物化學及分子生物學為基礎，探討植物基因轉殖之理論、方法與應用方向，並闡述國際上轉殖植物 (transgenic plants) 之栽培、商業化現況及監督管理規範。本課程涵蓋植物組織培養(plant tissue culture)、代謝工程(metabolic engineering)及重組DNA技術 (recombinant DNA technology)，並針對最新植物基因轉殖文獻進行導讀及評論。 |
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課程要求 |
To discuss in the theory, methods, and reality of plant gene transfer, and the
application of transgenic plants. |
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預期每週課後學習時數 |
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Office Hours |
另約時間 |
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指定閱讀 |
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參考書目 |
General
1. Bennett AB & O'Nell SD (eds) (1990) Horticultural Biotechnology. Wiley-
Liss, Inc., New York. 387pp.
2. Chawla HS (2002) Introduction to Plant Biotechnology. 2nd ed. Science
Publishers, Inc., Enfield, NH. 538pp.
3. Clark DP (ed) (2005) Molecular Biology. Elsevier Academic Press. 784pp
4. Collins GB & Shepherd RJ (eds) (1996) Engineering Plants for Commercial
Products and Applications. Annals of the New York Academy of Sciences, Volume
792. 183pp.
5. Dennis ES, Hohn B, Hohn T, King PJ, Schell J & Verma DPS (eds) (1991) Plant
Gene Research. Springer-Verlag Wien, New York. 166pp.
6. Fowler MW & Warren GS (eds) (1992) Plant Biotechnology. Pergamon Press.
366pp.
7. Gibson G & Muse SV (2002) A Primer of Genome Science. Sinauer Associates
Inc. Sunderland, Massachusetts. 347pp.
8. Glick BR & Pasternak JJ (2003) Molecular Biotechnology. American Society
for Microbiology, Washington, DC. 760 pp.
9. Grierson D & Covey SN (1988) Plant Molecular Biology. 2nd ed. Chapman and
Hall, New York. 233pp.
10. Grotewold E (2003) Plant Functional Genomics. Humana Press, Totowa, New
Jersey. 449pp.
11. Hiatt A (ed) (1993) Transgenic Plants. Marcel Dekker, Inc. 340pp.
12. Kung SD & Arntzen CJ (eds) (1989) Plant Biotechnology. Butterworths
Publishers. Boston. 423pp.
13. Lal R & Lal S (1990) Crop Improvement utilizing Biotechnology. CRC Press,
Inc. Boca Raton, Florida. 353pp.
14. Lamb CJ & Beachy RN (eds) (1990) Plant Gene Transfer. Alan R. Liss, Inc.
345pp.
15. Lewin, B. 2008. Genes IX. Jones and Bartlett Publishers, Inc. Sudbury,
Massachusetts, USA. 892 pp.
16. Lycett GW & Grieson D (1990) Genetic Engineering of Crop Plants.
Butterworths. 293pp.
17. Owen MRL & Pen J (1996) Transgenic Plants: A Production Sysetm for
Industrial and Pharmaceutical Proteins. John Wiley & Sons Ltd, New York. 348pp.
18. Ramawat KG (ed) (2004) Biotechnology of Medicinal Plants – Vitalizer and
Therapeutic. Science Publishers, Inc. 302pp.
19. Slater A, Scott NW & Fowler MR (2008) Plant Biotechnology – The Genetic
Manipulation of Plants. 2nd ed. Oxford University Press. 376pp.
20. Tropp BE (2008) Molecular Biology – Genes to Proteins. 3rd ed. Jones and
Bartlett Publishers, Inc. 1000pp.
21. You CB, Chen ZL & Ding Y (1992) Biotechnology in Agriculture. Proceedings
of the First Asia-Pacific Conference on Agricultural Biotechnology (Beijing,
China, 1992). Kluwer Academic Publishers, Dordrecht, Boston, London. 519pp.
Lab manual
1. Channarayappa (2007) Molecular Biotechnology – Principles and Practices.
Universities Press. Hyderabad, India. 1217pp.
2. Draper J, Scott R, Armitage P & Walden R (eds) (1988) Plant Genetic
Transformation and Gene Expression - A laboratory manual. Blackwell Scientific
Publications. Oxford. 355pp.
3. Gartland KMA & Davey MR (eds) (1995) Agrobacterium Protocols. Humana Press.
Totowa, New Jersey. 417pp.
4. Lindsey K (ed) (1993) Plant Tissue Culture Manual - Supplement 3. Kluwer
Academic Publishers, Dordrecht, Boston, London.
5. Martinez-Zapater JM & Salinas J (1998) Arabidopsis Protocols. Humana Press.
Totowa, New Jersey. 440pp.
6. Shaw CH (ed) (1988) Plant Molecular Biology -A practical approach. IRL
Press. Oxford. Washington DC. 313pp.
7. Tagu D & Moussard C (ed) (2006) Techniques for Molecular Biology. Science
Publishers, Enfield, NH,USA. 227pp.
8. Weigel D & Glazebrook J (2002) Arabidopsis: A laboratory manual. Cold
Spring Harbor Laboratory Press. New York. 354pp. |
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
期中考 |
33% |
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2. |
報告 |
34% |
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3. |
作業 |
0% |
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4. |
隨堂測驗 |
0% |
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5. |
文獻導讀或期末考 |
33% |
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週次 |
日期 |
單元主題 |
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第1週 |
09/15 |
Introduction |
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第2週 |
09/22 |
中秋節 |
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第3週 |
09/29 |
Possible risks and safety assessments of transgenic plants |
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第4週 |
10/06 |
Agrobacterium tumefaciens |
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第5週 |
10/13 |
Agrobacterium-mediated transformation |
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第6週 |
10/20 |
DNA-mediated gene transfer |
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第7週 |
10/27 |
Alternative DNA transfer methods |
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第8週 |
11/03 |
Virus vectors and their potential application in plant genetic engineering |
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第9週 |
11/10 |
Genetic manipulation of the chloroplast genome |
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第10週 |
11/17 |
期中考 |
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第11週 |
11/24 |
Reporter genes |
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第12週 |
12/01 |
Antisense RNA technology and ribozyme |
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第13週 |
12/08 |
Manipulation of floral pigmentation genes |
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第14週 |
12/15 |
Herbicide resistance |
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第15週 |
12/22 |
Strategies for disease resistance in plants |
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第16週 |
12/29 |
Gene silencing and RNA interference |
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第17週 |
01/05 |
Molecular strategy to modify genes involved in ethylene biosynthesis |
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第18週 |
01/12 |
期末考 |
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